OUR PRODUCTS
SAFE customize diets with tamoxifen to activate CreER. We also make doxycycline diets for tet regulated systems. The Cre/lox site-specific recombination system has emerged as a mean for the generation of conditional somatic mouse GMO. This method allows one to control gene activity in space and time in almost any tissue of the mouse.
| Code | Diets | Advantages | Daily dose of tamoxifen in mg / kg body weight (20 g BW and 3.2 g ingested) |
|---|---|---|---|
| E8220 Version 0352 | A04 at 0.25g/Kg TAMOXIFEN | Standard diet | 40 |
| E8220 Version 0366 | A04 at 0.5g/Kg TAMOXIFEN | Standard diet | 80 |
| U8404 Version 0008 | 150 at 0.25g/Kg TAMOXIFEN | Without soy and without alfalfa | 40 |
| U8404 Version 0009 | 150 at 0.5g/Kg TAMOXIFEN | Without soy and without alfalfa | 80 |
| U8978 Version 0056 | AIN-93G at 0.25g/Kg TAMOXIFEN | Contains few isoflavones | 40 |
| U8978 Version 0057 | AIN-93G at 0.5g/Kg TAMOXIFEN | Contains few isoflavones | 80 |
| Doses modified according to your needs. | |||
All these diets have tamoxifen. SAFE supplies the tamoxifen.
The Tamoxifen citrate form is different with only 66% tamoxifen, both forms are effective but the citrate form is not provided by SAFE.
Why in diets?
Rodents are sensitive to metallic tamoxifen taste. This induce a low intake (3g diet/day), and a potential weight loss (10%) on the first days. To limit these effects, 5% sugar is added as a flavor enhancer at all tamoxifen diets.
Nevertheless, feed aversion may still occur, also it’s recommended to make a daily observation of animal tolerance. For the welfare of rodents and for limit weight loss, the protocol should alternate 2 weeks of treatment and one week off. Prefer standard diet on weekends.
| Dosing via | Advantages | Disadvantages |
|---|---|---|
| Diet | Not very stressful for animals Little work | The dose depends on the eating behavior Induction is longer than IP |
| Drinking water | Not very stressful for animals Little work | Insoluble in water <0.1% at 20 ° C The dose depends on the consumption behavior Induction is longer than IP |
| Intraperitoneal injection (IP) | Accurate dosing | Stressful method for rodents that requires an expert manipulator |
Useful informations
- Store diet at +4°C and plan to use it within BBD (six months)
- Keep away of natural light, Tamoxifen is sensitive to UV
- Minimum order quantity is 10 kg
- Irradiation 10 kGy or 25 kGy is possible on request
- >> Precautionary principles <<
Learn more about the model tamoxifen :
- See at Methods in Molecular Biology 530:343-63 · February 2009 DOI: 10.1007/978-1-59745-471-1_18 PubMed
- Tamoxifen is a selective estrogen response modifier (SERM), protein kinase C inhibitor and anti-angiogenetic factor, meaning it is a mixed estrogen agonist and antagonist.
- Pure tamoxifen dose commonly used are 250 mg or 500 mg.
- Tamoxifen diets contains Tamoxifen. The molecule is diluted in diets but Personal Protective Equipment is recommended: Eyeshields, Lab coat, Gloves, Face particle respirator or Mask