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SAFE customizes diets with doxycycline hyclate for Tet regulated systems. SAFE also produces tamoxifen to activate CreER.
Doxycycline hyclate is applied in tetracycline (doxycycline)-inducible gene expression as a stable switch for controlling transcriptional activation, also known as a Tet-On/Tet-Off system with GMO mice doxycycline sensitive.

CodeDiets Diets advantagesDaily doxycycline dose
mg/kg Body Weight
(20-25 g BW & 3g intake)
E8200 Version 0092 A03 at 0.2g/Kg Doxycycline HyclateStandard Diet40
E8200 Version 0115A03 at 0.625g/Kg Doxycycline HyclateStandard Diet125
E8200 Version 0001A03 at 1g/Kg Doxycycline HyclateStandard Diet200
E8200 Version 0100A03 at 10g/Kg Doxycycline Hyclate Standard Diet2000
E8404 Version 0010 150 at 0.2g/Kg Doxycycline Hyclate Soya and alfalfa free40
E8404 Version 0002150 at 0.625g/Kg Doxycycline HyclateSoya and alfalfa free125
E8404 Version 0011150 at 1g/Kg Doxycycline HyclateSoya and alfalfa free200
E8404 Version 0012150 at 10g/Kg Doxycycline HyclateSoya and alfalfa free2000
U8978 Version 0058AIN 93-G at 0.2g/Kg Doxycycline HyclateLow Isoflavones40
U8978 Version 0059AIN 93-G at 0.625g/Kg Doxycycline HyclateLow Isoflavones125
U8978 Version 0060AIN 93-G at 1g/Kg Doxycycline HyclateLow Isoflavones200
U8978 Version 0061 AIN 93-G at 10g/Kg Doxycycline Hyclate Low Isoflavones2000
Customized dose in custom diet according to your request.

All these diets have Doxycycline Hyclate. SAFE supplies the Doxycycline Hyclate. Doxycycline hyclate is a semisynthetic tetracycline, this use is authorized at subantimicrobial doses.

Why in diets?

The benefit of doxycycline diet versus water delivery is the better stability due to the low degradation rate of doxycycline hyclate. This pelleted form reduce exposure to light, for water administration it is preferable to use amber water bottles since tetracycline and doxycycline are light sensitive. The water administration includes 2 bias: the risk of dehydration (molecule taste) and the need to use sucrose in the water.

Dosage via AdvantagesDisadvantages
ChowLess stressful for the animals
Less labor
Dose depends on eating behaviour
Drinking waterLess stressful for the animals
Concentrated solution can be frozen in 5 ml aliquots
Tetracycline and doxycycline are light sensitive, use amber water bottles
Dose depends on drinking behaviour
Use sucrose in the water
Daily change of water bottles
Intraperitoneal Injection (IP)Reliable dosage
Only necessary for very sick mice
Stressful method for the rodents
Requires a qualified handler

Useful informations

  • Store diet at +4°C and plan to use it within BBD (six months)
  • The doxycycline chow change is every 1 week.
  • Keep away of natural light, Doxycycline is sensitive to UV
  • Minimum order quantity is 10 kg
  • Irradiation 10kGy, 25kGy or 45kGy is possible on request
  • >>  Precautionary principles  <<

In order to optimize the 6 months of DLUO for your protocols, doxycycline diets are manufactured on request, that allows to customize them to the exact concentration needed. Custom diets requests include: base diet, concentration of doxycycline hyclate, food coloring.

If your protocol requires fluorescent imaging, consider using purified diet for improve imaging clarity.

Learn more about the Doxycycline Hyclate model

  • Christopher Cawthorne, Ric Swindell, Ian J. Stratford, Caroline Dive, and Arkadiusz Welman; Comparison of Doxycycline Delivery Methods for Tet-Inducible Gene Expression in a Subcutaneous Xenograft Model; J Biomol Tech. 2007 Apr; 18(2): 120–123; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2062538/
  • Paul Wassarman, Philippe M Soriano; Guide to Techniques in Mouse Development, Part B: Mouse Molecular Genetics; Academic Press, 24 sept. 2010; ISBN 0123848814, 9780123848819
  • Gossen M, Freundlieb S, Bender G, Müller G, Hillen W, Bujard H (1995). „Transcriptional activation by tetracyclines in mammalian cells“. Science (journal). 268 (5218): 1766–1769. doi:10.1126/science.7792603. PMID 7792603.
  • Orth P, Schnappinger D, Hillen W, Saenger W, Hinrichs W (2000). „Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system“. Nature Structural & Molecular Biology. 7 (3): 215–219. PMID 10700280.
  • Tet-On and Tet-Off are registered trademarks of Clontech Laboratories, Inc. in the United States.
  • Bujard, Hermann; M. Gossen (1992). „Tight Control of Gene Expression in Mammalian Cells by Tetracycline-Responsive Promoters.“. Proc. Natl. Acad. Sci. U.S.A. 89 (12): 5547–51. doi:10.1073/pnas.89.12.5547. PMID 1319065.
  • Allen, Nicholas D.; Antonius Plagge; Gavin Kelsey (2000). „Directed Mutagenesis in Embryonic Stem Cells“. Mouse Genetics and Transgenics: 259–263.
  • Urlinger, Stefanie; Baron, Udo; Thellmann, Marion; Hasan, Mazahir T.; Bujard, Herman; Hillen, Wolfgang (2000). „Exploring the sequence space for tetracycline-dependent transcriptional activators: Novel mutations yield expanded range and sensitivity.“. Proc. Natl. Acad. Sci. U.S.A. 97 (14): 7963–8. doi:10.1073/pnas.130192197. PMID 10859354.
  • Zhou, X.; Vink, M.; Klave, B.; Berkhout, B.; Das, A.T. (2006). „Optimization of the Tet-On system for regulated gene expression through viral evolution.“. Gene Ther. 13 (19): 1382–1390. doi:10.1038/sj.gt.3302780. PMID 16724096.
  • Sohal DS, Ngheim M, Crackower MA, Witt SA, Kimball TR, Tymitz KM, Penniger JM, Molkentin JD (2001) Temporally regulated and tissue-specific gene manipulations in the adult and embryonic heart using tamoxifen-inducible Cre protein. Circ Res 89:20-25
  • Moullan N, Mouchiroud L, Wang X, Ryu D, Williams EG, Mottis A, Jovaisaite V, Frochaux MV, Quiros PM, Deplancke B, Houtkooper RH, Auwerx J (2015). „Tetracyclines Disturb Mitochondrial Function across Eukaryotic Models: A Call for Caution in Biomedical Research.“. Celll Reports. 10 (10): 1681–91. doi:10.1016/j.celrep.2015.02.034. PMID 25772356.
  • Chatzispyrou IA, Held NM, Mouchiroud L, Auwerx J, Houtkooper RH (2015). „Tetracycline antibiotics impair mitochondrial function and its experimental use confounds research.“. Cancer Research. 72 (21): 4446–9. doi:10.1158/0008-5472.CAN-15-1626. PMID 26475870.

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